Produktinformationen
APC Resistance Kit
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APC Resistance Kit is a plasma based functional assay for the determination of resistance to activated protein C caused by the Factor V -Leiden mutation (FV:Q506).
Test principle
APC Resistance Kit is a plasma based functional clotting assay and differs from other functional APC resistance tests by acting specifically on the prothrombinase complex level. It is based on a FV-dependent prothrombin activator isolated from snake venom. Robustness and specificity of the assay is enhanced by elimination of possible disturbing influences by factors upstream the coagulation cascade and independency from calcium. Interference from UFH, LMWH and pentasaccharides in the blood sample is precluded by a heparin inhibitor added to reagents 1 and 2. Sample plasma is pre-diluted with reagent 4 (dilution plasma) and incubated at 37 °C with FV activator from snake venom (RVV-V from Daboia russelli). Coagulation is triggered by the addition of a FV dependent prothrombin activator from snake venom from Notechis scutatus scutatus in the absence of calcium. The clotting times are recorded and the ratios (clotting time in the presence of APC / clotting time in the absence of APC) are calculated.
Reagents and stability (opened/ reconstituted)
The expiry date printed on the labels is only applicable to storage of the unopened containers at 2-8 °C.
| Reagenz | Menge | Haltbarkeit / Hinweis |
|---|---|---|
| R1 APC / RVV-V (+APC) | 3 x 2 mL | 14 days at 2–8°C 24 h at 15-25°C 6 month at < -20°C |
| R2 RVV-V (-APC) | 3 x 2 mL | 14 days at 2–8°C 24 h at 15-25°C 6 month at < -20°C |
| R3 PTA Reagent | 3 x 4 mL | 14 days at 2–8°C 24 h at 15-25°C 6 month at < -20°C |
| R4 Dilution Plasma | 3 x 2 mL | 14 days at 2–8°C 24 h at 15-25°C 6 month at < -20°C |
| C1 FV-L Negative Control | 1 x 1 mL | 8 days at 2–8°C 8 h at 15-25°C 6 month at < -20°C |
| C2 FV-L Heterozygous Control | 1 x 1 mL | 8 days at 2–8°C 8 h at 15-25°C 6 month at < -20°C |
| Artikelnummer |
|---|
| 5344510 |