Produktinformationen
COALIZA Anticardiolipin
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An enzyme linked immunosorbent assay (ELISA) for the quantitative determination of IgG and IgM anticardiolipin antibodies (aCL) in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (antiphospholipid antibody syndrome).
Test principle
Samples, controls and calibrators are incubated in micro wells, which have been coated with cardiolipin, allowing anticardiolipin antibodies (aCL) to bind. Unbound substances are removed by washing. An HRP conjugated detecting antibody, specific for human IgG or IgM is added and binds to the cardiolipin/aCL antibody complex. After a washing step, added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of IgG or IgM anticardiolipin antibodies in the sample.
Reagents and stability (after opening)
| Reagenz | Menge |
|---|---|
| Micro wells | 12 x 8 |
| Sample diluent | 1 vial |
| aCL IgG calibrator serum | 3 vials |
| aCL IgM calibrator serum | 3 vials |
| aCL IgG positive control | 1 vial |
| aCL IgM positive control | 1 vial |
| aCL normal control | 1 vial |
| anti IgG-conjugate | 1 vial |
| anti IgM-conjugate | 1 vial |
| Substrate | 1 vial |
| Stop solution | 1 vial |
| Washing buffer (concentrate) | 1 vial |
When stored at 2-8°C the reagents remain stable until the expiry date printed on the vials.
| Anzahl der Bestimmungen | Artikelnummer |
|---|---|
| 96 | 023-001 |