A chromogenic method for the determination of α₂-macroglobulin activity in plasma.

Test principle

α₂M + Trypsin (excess) ⟶ [α₂M • Trypsin] + Trypsin (residual)

Trypsin (residual) + SBTI (excess) ⟶ [SBTI • Trypsin (residual)]

Substrate [α₂M • Trypsin]⟶ Peptide + pNA

Reagents and stability (after opening)

Reagenz	Menge	Haltbarkeit / Hinweis
Trypsin	1 vial	4 hours at 4°C 6 months at -20°C
Trypsin-substrate	1 vial	8 hours at 4°C 6 months at -20°C
Soy Bean Trypsin Inh.	1 vial	8 hours at 4°C 6 months -20°C
Buffer concentrate	1 vial	see expiry date
Standard plasma	1 vial	8 hours at 4°C

Artikelnummer
0041