Capture and detecting antibodies for the construction of an ELISA for the quantitative determination of thrombin-heparin cofactor II complex.

Test principle

Micro wells are coated with anti-thrombin antibodies (capture antibody), to which the thrombin-heparin cofactor II complex from the sample binds. Unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added, which bind to the thrombin-heparin cofactor II / capture antibody complex. After a washing step, added enzyme reacts with the conjugate. The intensity of the resulting colour is proportional to the thrombin-heparin cofactor II complex content in the sample.

Reagents and stability (after opening)

When stored at -20°C the reagents remain stable until the expiry date printed on the vial.