Product information
ELISA ADAMTS-13-Inhibitor
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Complete ELISA kit for the determination of human antibodies, directed against ADAMTS-13 and inhibiting its activity.
Test principle
The samples are incubated in micro wells coated with recombinant ADAMTS-13. After binding of anti-ADAMTS-13-autoantibodies to the immobilized protein, unbound substances are removed by washing. A peroxidase conjugated anti-human-IgG-antibody is added and binds to the already bound anti-ADAMTS-13-autoantibodies. After a washing step, added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the anti-ADAMTS-13-autoantibody content of the sample
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8/6x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C |
| Controls high, low | each 1 vial | 6 months at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | 5450401 |
| 48 | 5450451 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA ADAMTS-13 Inhibitor Calibrator Set | 5 x 0.5 ml | 5450461 |
| ELISA ADAMTS-13 Inhibitor Control Set | 2 x 0.5 ml | 5450463 |