Product information
ELISA vWF:AG
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Complete ELISA kit for quantitative determination of the von Willebrand-factor (vWF) in plasma.
Test principle
The diluted samples are incubated in micro wells that are coated with polyclonal antibodies against vWF together with the conjugate. After the vWF has been bound to the capture antibodies and the peroxidase-conjugated detecting antibodies to the vWF/capture antibody-complex unbound substances are removed in a washing step. Added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of vWF in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | expiry date 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vials | 2 months at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C 8 hours at RT |
| Controls high, low | each 1 vial | 6 months at -20°C 8 hours at RT |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB-Substrate | 1 vial. | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date |
| Number of determinations | Article number |
|---|---|
| 96 | 5450201 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA vWF:AG Calibrator Set | 5 x 0,5 ml | 5450210 |
| ELISA vWF:AG Elisa Antigen Control Set | 2 x 0,5 ml | 5450212 |