Product information
ELISA ADAMTS-13 Activity
Automatically transferred from the PDF. Please compare details with the original document.
Chromogenic complete ELISA-kit for determination ADAMTS-13 activity in human plasma.
Test principle
GST-vWF73 substrate is incubated in micro wells coated with monoclonal anti-GST-antibodies. After a washing step the diluted samples are added into the wells and the plate is incubated. ADAMTS-13 cleaves the GST-vWF73 substrate. Unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added, which detects the cleavage site on GST-vWf73. After a second washing step, added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the activity of ADAMTS-13 in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Reaction buffer | 1 vial | 6 months at 2-8°C |
| Calibrators 1-6 | each 1 vial | 6 months at -20°C |
| Controls high, low | each 1 vial | 6 months at -20°C |
| GST-VWF73-substrate | 2 vials | 6 weeks at–20°C |
| Conjugate | 1 vial | 4 months at 2-8°C |
| TMB-substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | 5450701 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA ADAMTS-13 Activity Calibrator Set | 6 x 0.5 ml | 5450761 |
| ELISA ADAMTS-13 Activity Control Set | 2 x 0.5 ml | 5450763 |