Product information
VisuLize® Factor VIII Antigen Kit
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Complete ELISA kit for the quantitative determination of factor VIII Antigen. For in vitro diagnostic determination of factor VIII in plasma as well as in factor VIII-concentrates.
Test principle
The samples are incubated in micro wells coated with antibodies against factor VIII. After binding to the capture antibody unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added and binds to the factor VIII/Capture Antibody-complex. After a washing step added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the factor VIII content in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 6x16 | expiry date |
| Calibration plasma | 2x1ml | 4 hours at RT 30 days at -20°C |
| Control plasma A | 2x1ml | 4 hours at RT 30 hours at –20°C |
| Control plasma B | 2x1ml | 4 hours at RT 30 days at –20°C |
| Wash buffer conc | 1x50ml | expiry date |
| Sample buffer conc | 3x20ml | expiry date |
| Conjugate | 1x12ml | expiry date |
| Substrate | 1x12ml | expiry date |
| Stop solution | 1x12ml | expiry date |
| Number of determinations | Article number |
|---|---|
| 96 | FVIII-AG |