Product information
ELISA vWF:CBA
Automatically transferred from the PDF. Please compare details with the original document.
Complete ELISA kit for quantitative determination of high molecular von Willebrand-factor (vWF) multimers, based on the collagen binding activity.
Test principle
The samples are incubated in micro wells coated with a human collagen (Type III). After binding of the vWF-multimers to the collagen unbound substances are removed in a washing step. A peroxidase-conjugated detecting antibody is added and binds to the vWF/collagen-complex. After a washing step an added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of high molecular vWF-multimers in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C 8 hours at RT |
| Control high, low | each 1 vial | 6 months at -20°C 8 hours at RT |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB-Substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date |
| Number of determinations | Article number |
|---|---|
| 96 | 5450301 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA vWF:CBA Calibrator Set | 5 x 0,5 ml | 5450310 |
| ELISA vWF:CBA Control Set | 2 x 0,5 ml | 5450312 |