Product information
ELISA ADAMTS-13 Antigen
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Complete ELISA kit for the determination of human ADAMTS-13
Test principle
The diluted samples are incubated in micro wells coated with a monoclonal anti-ADAMTS-13 antibody. After binding of ADAMTS-13 to the capture antibody, unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added and binds to the ADAMTS-13 / capture antibody complex. After a washing step, added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of ADAMTS-13 in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C |
| Controls high, low | each 1 vial | 6 months at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | 5450601 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA ADAMTS-13 Antigen Calibrator Set | 5 x 0.5 ml | 5450661 |
| ELISA ADAMTS-13 Antigen Control Set | 2 x 0.5 ml | 5450663 |