Product information
ELISA t-PA Actibind®
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Complete ELISA-kit for quantitative determination of the activity of human tissue-type plasminogen activator (t-PA) as well as the total human t-PA concentration in human plasma.
Test principle
The diluted samples are incubated in micro wells coated with monoclonal anti-t-PA-antibodies. After binding of t-PA to the capture antibody, unbound substances are removed by washing and a mix of Glu-Plasminogen and plasmin-substrate is added. After a photometric activity determination the plate is washed leaving the bound t-PA on the plate. A secondary, peroxidase conjugated, anti-t-PA-antibody is added which recognizes bound t-PA. After a washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of t-PA in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Coated micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Standard | 1 vial | 6 months at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB-substrate | 1 vial | expiry date at 2-8°C |
| Plg.-act.-det. mix | 1 vial | 2 hours at 2-8°C 2 weeks at –70°C |
| Plg.-act.-det. mix dil. | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | TC16000 |