Product information
ELISA u-PA Actibind®
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Complete ELISA-kit for quantitative determination of the activity of human urokinase-type plasminogen activator (u-PA) as well as the total u-PA concentration in human plasma.
Test principle
The diluted samples are incubated in micro wells coated with monoclonal anti-u-PA-antibodies. After binding of u-PA to the capture antibody, unbound substances are removed by washing and a mix of Glu-plasminogen and plasmin-substrate is added. After a photometric activity determination the plate is washed, leaving the bound u-PA on the plate. A secondary, peroxidase conjugated, anti-u-PA-antibody is added which recognizes bound u-PA. After a washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of u-PA in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Coated micro wells | 12x8 | 8 weeks at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 4-8 weeks at 2-8°C |
| Dilution buffer | 1 vial | 4-8 weeks at 2-8°C |
| Standard | 1 vial | 8 weeks at -70°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| Conjugate Dil. Buffer | 1 vial | 4-8 weeks at 2-8°C |
| TMB-substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date |
| Plg.-act.- det. mix | 1 vial | 4 hours at RT 8 weeks at –70°C |
| Plg.-act.- det. mix dil. | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | TC16010 |