Product information
ELISA Fibronectin
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Complete ELISA kit for the quantitative determination of human fibronectin.
Test principle
The samples are incubated in micro wells coated with an anti-fibronectin antibody. After binding of fibronectin to the capture antibody, unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added and binds to fibronectin /capture antibody complex. After a washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of fibronectin in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | 8 weeks at 4°C |
| Diluent buffer | 3 vials | 4-8 weeks at 4°C |
| Calibrator | 1 vial | 4 hours at RT 8 weeks at –70°C |
| Conjugate | 1 vial | 4 hours at RT |
| Wash buffer (12.5 x) | 1 vial | 4-8 weeks at 4°C |
| TMB-substrate | 1 vial | until expiry date at 2-8°C |
| Stop solution | 1 vial | until expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | TC12030 |