Product information
ELISA ADAMTS-13 Activity/Antigen
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Complete fluorogenic ELISA-kit for the determination of ADAMTS-13 activity and antigen concentration in human plasma.
Test principle
The undiluted samples are incubated in micro wells coated with monoclonal anti-ADAMTS-13-antibodies. After binding of ADAMTS-13 to the capture antibody, unbound substances are removed by washing. Added activity substrate reacts with the bound ADAMTS-13 and the resulting fluorescence is proportional to the ADAMTS-13 activity of the sample. After a washing step, a peroxidase conjugated detecting antibody is added and binds to the ADAMTS-13 /capture antibody complex. After a second washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the antigen content of ADAMTS-13 in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8/6x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 month at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C |
| Controls high, low | each 1 vial | 6 months at -20°C |
| Activity substrate | 1/2 vial(s) | 2 months at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| Antigen substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | 2 years at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 2 x 48 | 5450501 |
| 48 | 5450551 |
Also available
| Product | Pack size | Article number |
|---|---|---|
| ELISA ADAMTS-13 Calibrator Set | 5 x 0.5 ml | 5450561 |
| ELISA ADAMTS-13 Control Set | 2 x 0.5 ml | 5450563 |