Product information
ELISA u-PA Antigen
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Complete sandwich ELISA kit for quantitative determination of human urokinase (u-PA) in plasma and other biological fluids.
Test principle
The diluted samples are incubated in micro wells coated with monoclonal anti-u-PA-antibodies. After binding of u-PA to the capture antibody, unbound substances are removed by washing. A peroxidase conjugated detecting antibody is added, which recognizes bound u-PA. After a washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of u-PA in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Coated micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Standard | 1 vial each | 6 months at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB-substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date |
| Number of determinations | Article number |
|---|---|
| 96 | TC12010 |