Product information
Technochrom Protein C 2G
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The Technochrom Protein C 2G is an in vitro diagnostic reagent kit for the quantitative, WHO standardized determination of protein C activity as an aid to diagnosis and monitoring of congenital or acquired Protein C deficiencies in patients with thrombotic tendencies by means of automated or manual chromogenic methods.
Test principle
Technochrom Protein C 2G is a two-stage, kinetic chromogenic assay of APC activity. The first stage involves activation of PC in diluted plasma by a PC-specific activator purified from the venom of the Southern Copperhead snake (Agkistrodon contortrix contortrix). After an incubation period, the resultant APC is reacted with an APC-specific chromogenic substrate, generating the coloured cleavage product,p-nitroaniline. The colour intensity is directly proportional to the PC concentration, which is measured spectrophotometrically.
Reagents and stability (reconstituted)
Unopened reagents are stable until the stated expiration date indicated on the label at 2-8 °C. Do not freeze!
Store reagents upright in their packaging.
| Reagent | Quantity | Stability / note |
|---|---|---|
| Substrate (Pyr-Pro-Arg-pNA, caesium chloride), lyophilized | 3 x 4 mL | 2 days at 2-8 °C 8 h at 15-25 °C |
| RE Reagent, lyophilized | 3 x 1 mL | 2 days at 2-8 °C 8 h at 15-25 °C |
| Number of determinations | Article number |
|---|---|
| 60 | 5341014 |