Product information
ELISA vWF:CBA Type VI
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Complete ELISA kit for quantitative determination of high molecular von Willebrand-factor (vWF) multimers, based on the collagen binding activity.
Test principle
The samples are incubated in micro wells coated with a human collagen (Type VI). After binding of the vWF-multimers to the collagen unbound substances are removed in a washing step. A peroxidase-conjugated detecting antibody is added and binds to the vWF/collagen-complex. After a washing step an added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of high molecular vWF-multimers in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Micro wells | 12x8 | expiry date at 2-8°C |
| Wash buffer (10-fold) | 1 vial | 6 months at 2-8°C |
| Incubation buffer | 1 vial | 2 months at 2-8°C |
| Calibrators 1-5 | each 1 vial | 6 months at -20°C 8 hours at RT |
| Control high, low | each 1 vial | 6 months at -20°C 8 hours at RT |
| Conjugate | 1 vial | 6 months at 2-8°C |
| TMB-Substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date |
| Number of determinations | Article number |
|---|---|
| 96 | 5450321 |