Chromogenic method for quantitative determination of factor XIII-activity in human plasma.

Test principle

Thrombin activates factor XIII to factor XIIIa. Coagulation of the simultaneously formed fibrin is prevented by addition of a fibrin polymerization inhibitor. Activated factor XIII links the amine substrate glycine-ethylene-ester with the glutamine residual of the specific peptide substrate. Ammoniac is released. In the measurement reaction the released ammoniac is demonstrated in a glutamate dehydrogenase catalyzed NADPH dependent reaction. The consumption of NADPH is spectrometrically measured by decrease of extinc-tion at 340 nm. Within one time interval the extinction decrease is proportional to the factor XIII activity.

Reagents FXIII Activator Reagent 3 vial FXIII Detections Reagent 3 vial FXIII NADPH solution 3 vials Inhibitor Reagent 3 vials Stabilizer solution 1 vial

Reagent stability 3 days at 2-8°C after reconstitution 3 months at –20°C

Analyzer applications: on request