Product information
ELISA t-PA/PAI-1-Complex
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Complete Sandwich ELISA kit for quantitative determination of human t-PA/PAI-1 complexes in plasma and other biological fluids.
Test principle
The samples are incubated in micro wells coated with monoclonal anti-t-PA-antibodies (mouse). After binding of t-PA/PAI-1 complexes to the capture antibody, unbound substances are removed by washing. A monoclonal peroxidase conjugated detecting anti-PAI-1-anti-body is added and binds to the t-PA/PAI-1 complex. After a washing step, added substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of t-PA/PAI-1 complexes in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Coated micro wells | 12x8 | 8 weeks at 2-8°C |
| Wash buffer (12.5-fold) | 1 vial | 4-8 weeks at 2-8°C |
| Dil. buffer (2.5-fold) | 1 vial | 4-8 weeks at 2-8°C |
| Standard | 1 vial | 8 weeks at -20°C |
| Conjugate | 1 vial | 6 months at 2-8°C |
| Conjugate-Dil. buffer | 1 vial | 4-8 weeks at 2-8°C |
| TMB-substrate | 1 vial | expiry date at 2-8°C |
| Stop solution | 1 vial | expiry date at 2-8°C |
| Number of determinations | Article number |
|---|---|
| 96 | TC12080 |